Polymorphism of peripheral blood T cell receptor β chain variable region in patients with clinically isolated syndrome

Abstract

Objective To investigate the polymorphism of T cell receptor β chain variable region (TCRVβ) in peripheral blood of clinically isoiated syndrome (CIS) patients. To preliminarily predict the specific TCRVβ fragment of CIS and its diagnostic value for CIS. Methods Hospitalized patients of Beijing Xuanwu Hospital from September 2012 to March 2014, were divided into 2 groups including CIS group (experimental pateints, n=30) and normal control group (healthy volunteers, n=30). The routine immune indexes and expression of TCRVβ subfamilies were detected by flow cytometry. CELL Quest Pro 5.1 software was used to analyze 24 subfamilies of TCRVβ. Calculated the statistic of amplification rate, amplification number and amplification fraction of the total 24 subfamilies of TCRVβ. Results Compared to the normal control group, the quantitative expression of CD4+TCRVβ2, 4, 5.2, 5.3, 7.1, 8, 14, 17, 20, 21.3 and CD8+TCRVβ4, 14 were significantly expanded in CIS group, CD4+TCRVβ11 and CD8+TCRVβ9, 11 were significantly reduced in CIS group (P<0.05or P<0.01). The amplification rate of CD4+TCRVβ2 (P=0.002) and CD8+TCRVβ14 (P=0.010) in CIS group were higher than those in normal control group, but there was no significant difference in the other subfamilies. There was no statistical difference in the expansion rate of the TCRVβ between CIS group and normal control group. The amplification number (P= 0.001) and amplification fraction (P=0.006) of the TCRVβ in CIS group were significantly higher than the normal control group. Receiver operating characteristic curve (ROC) analysis indicated that the area under thecurve (AUC)of CD4+TCRVβ4 in the diagnosis of CIS was 0.719 (95%CI:0.592-0.851, P=0.003). The sensitivity was 83.33%,the specificity was 56.67%. The AUC of CD4+TCRVβ14 in the diagnosis of CIS was 0.713 (95%CI: 0.581-0.845, P=0.005). The sensitivity was 70%, the specificity was 56.23%. The AUC of CD8+TCRVβ4 in the diagnosis of CIS was 0.727(95%CI:0.600-0.854, P=0.002). The sensitivity was 93.33%, the specificity was 50%. The three fragments have medium diagnostic value for CIS. Conclusions Although the expression levels of CD4+TCRVβ4, CD4+TCRVβ14 and CD8+TCRVβ4 in peripheral blood are increased in CIS group, their diagnostic value is limited.

DOI：10.3969/j.issn.1672-6731.2020.09.005