Evaluation on lipid detection of CardioChek PA blood analyzer and its application in lipid screening in high-risk stroke group

Abstract

Objective To evaluate the reliability of CardioChek PA portable blood analyzer in the detection of blood lipid and to explore the feasibility and accuracy of its application in lipid screening in high-risk stroke group. Methods According to the profile of the National Committee for Clinical Laboratory Standards of Method Comparison and Bias Estimation Using Patient Samples (NCCLS-EP9-A), two different biochemical analysis systems, CardioChek PA blood analyzer (testing system) and Siemens ADVIA2400 automatic analyzer (reference system), were used respectively to test lipids including total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) to obtain the correlation coefficient and the linear regression equation as well as predicted bias. Based on the comparison, CardioChek PA blood analyzer was used to detect blood lipid of 1263 persons (more than 40 years old) at high-risk of stroke. Results The outcomes of TC, TG, HDL-C and LDL-C between CardioChek PA blood analyzer and Siemens ADVIA2400 automatic analyzer had significant correlations (r > 0.975, P < 0.05) and error allowance at different medical decision levels were all between 95% confidence interval (CI) of predicted bias, indicating that there were comparability of TC, TG, HDL-C and LDL-C between two detecting systems and the outcomes were acceptable. Moreover, there were
347 cases (27.47% ) with abnormal TC, 348 cases (27.55% ) with abnormal TG, 62 cases (4.91% ) with abnormal HDL-C and 441 cases (34.92%) with abnormal LDL-C among 1263 persons. Conclusion There is comparability of TC, TG, HDL-C and LDL-C between CardioChek PA blood analyzer and ADVIA2400 biochemical automatic analyzer and the outcomes of CardioChek PA are accurate and confident, thus CardioChek PA can be used in epidemiological study.