Application of automatic immunohistochemistry stainer in pathological diagnosis of glioma

Abstract

Objective To investigate and evaluate the Ki ⁃ 67 expression in glioma pathological diagnosis by Leica BOND⁃MAXTM automatic immunohistochemistry stainer. Methods Collect 120 glioma tissue paraffin blocks (30 cases for WHO Ⅰ, Ⅱ, Ⅲ, Ⅳ respectively) and perform histopathological examinations using EnVision by automatic stainer and conventional manual procedure. Results The interaction effects were presented between different WHO grades and staining methods. Statistical significance (P = 0.000, for all) was shown in the detection of Ki⁃67 labeling indexes by the two methods. The analysis of Ki⁃67 between and within WHOⅠand WHOⅡ had no statistical significance (P > 0.05, for all); however, the comparisons of Ki⁃67 labeling indexes between WHOⅠ,Ⅱand WHOⅢ,Ⅳ, between WHO Ⅲ and Ⅳ, within the same grade by using the two methods were all statistically significant (P < 0.05, for all), and the Ki ⁃ 67 labeling indexes by automatic stainer are higher than that by manual staining. Conclusion The standardization of immunohistochemistry by automatic stainer provides more reliable proof for histopathological diagnosis and contributes to the analysis of the grading and prognosis in gliomas．

DOI：10.3969/j.issn.1672⁃6731.2012.06.016