The effect of hyperbaric oxygen on survival time of C57 mice implanted with GL261 gliomas after chemotherapy with ACNU

Abstract

Objective To investigate the effect of hyperbaric oxygen (HBO) on the survival of C57 mice implanted with GL261 gliomas after chemotherapy with nimustine (ACNU). Methods Forty adult male C57 mice were divided into four groups: control, HBO, ACNU and HBO + ACNU randomly by SPSS 10, and planted with GL261 glioma cell suspension in their caudate nucleus to form tumors. From the 7th day after implantation, these groups (except control group) began to receive different treatments (HBO, ACNU and HBO + ACNU), in order to observe the effects of different treatments on the survival time of tumor bearing mice. Results There were no significant differences in the tumor volume, mass effect, severe compression of normal brain tissue and midline shift (F = 0.602, P = 0.618). The average survival time was significant with one⁃way ANOVA (F = 12.177, P = 0.000) and with the Kaplan⁃ Meier survival analysis of log⁃rank (χ2 = 13.604, P = 0.003), while multiple comparisons between groups showed HBO (χ2 = 0.365, P = 0.546) and single⁃time ACNU (χ2 = 0.884, P = 0.347) had no effect on the survival of mice; the effect of HBO + ACNU (χ2 = 9.962, P = 0.002) was better than that of HBO (χ2 = 6.925, P = 0.008) and single⁃ time ACNU (χ2 = 7.152, P = 0.007). Conclusion The HBO had no effect on survival time of C57 mice with implantation of GL261 and could not promote the growth of the GL261 glioma. However, combining the treatment with ACNU could extend the survival time of tumor bearing mice, suggesting that HBO could strengthen the therapeutic effects of ACNU．

DOI：10.3969/j.issn.1672⁃6731.2012.06.014