Study on expressions of heat shock 27⁃associated protein 1 and echinoderm microtubule⁃associated protein⁃like 5 in drug⁃resistant epilepsy

Abstract

Objective To observe the expressions of heat shock 27 ⁃ associated protein 1 (HSPBAP1) and echinoderm microtubule⁃associated protein⁃like 5 (EML5) in cerebrospinal fluid of drug⁃ resistant epilepsy, and to explore the value in early diagnosis of epilepsy. Methods According to the inclusion and exclusion criteria, 79 patients admitted in Department of Neurology, Hubei Xinhua Hospital and the First and Second Affiliated Hospital of Chongqing Medical University were divided into drug ⁃ resistant epilepsy group (n = 39) and non⁃epileptic control group (n = 40). Cerebrospinal fluid (every sample 4 ml) were collected by lumbar puncture specimens, and HSPBAP1 and EML5 were detected by sandwich enzyme⁃linked immunosorbent assays. SPSS 13.0 software was used for statistical analysis, and P ≤ 0.05 indicated significant differences. Results The expressions of HSPBAP1 and EML5 were 0.17 ± 0.03 and 0.13 ± 0.02 in drug⁃resistant epilepsy group, while were 0.10 ± 0.03 and 0.08 ± 0.02 in non⁃epileptic control group. There was significant difference between 2 groups (t = 3.239, P = 0.002; t = 3.294, P = 0.002, respectively). Conclusion The expressions of HSPBAP1 and EML5 were increased in drug ⁃ resistant epilepsy patients. This provides a new way for early diagnosis of drug⁃resistant epilepsy．

DOI：10.3969/j.issn.1672⁃6731.2012.05.014