Effect of hydroxyethyl starch 130/0.4 on blood⁃brain barrier in rats after global cerebral ischemia⁃reperfusion

Abstract

Objective To observe the effect of hydroxyethyl starch (HES) 130/0.4 on global cerebral ischemia ⁃ reperfusion in rats, and to investigate the underlying mechanism of HES130/0.4. Methods Forty ⁃ eight Sprague ⁃ Dawley (SD) rats were divided randomly into 4 groups, namely sham operation group (S group), ischemia⁃reperfusion group (IR group), normal saline (NS) + IR group (NS group) and HES130/0.4 + IR group (HES group). The blood gas analysis was performed during the experiment. The content of tumor necrosis factor⁃α (TNF⁃α), evans blue (EB), brain water content and nuclear factor⁃κB (NF⁃κB) activity in brain tissue were determined at 12 h after the experiment. Results No significant differences of arterial partial pressure of oxygen (PaO2) and partial pressure of carbon dioxide (PaCO2) in every group were seen at different observation time before and after operation (P > 0.05, for all). NF⁃κBp65 expression and Immunohistochemical Score (IHS) of brain tissue were all increased in IR group, NS group and HES group (P = 0.000, for all), while NF⁃κBp65 expression and IHS score in HES group were lower than those in other 2 groups (P = 0.000, for all). After ischemia⁃reperfusion injury, brain water content and blood⁃brain barrier (BBB) permeability in IR group and NS group were all significantly higher than those in HES group (P = 0.000, for all), while the 2 indices in HES group were all nearly approaching to normal range (P > 0.05, for all). Conclusion HES130/0.4 can alleviate ischemia ⁃ reperfusion BBB injury in ischemia⁃reperfusion rats. HES130/0.4 has cerebral protective effect, and the underlying mechanism may concerned with the decrease of TNF⁃α and NF⁃κB expression and attenuation of inflammatory injury.

DOI：10.3969/j.issn.1672-6731.2010.04.014