Association study on peroxisome proliferator ⁃ activated receptor γ 2 gene polymorphism and sporadic Alzheimer's disease

Abstract

Objective To study the association between peroxisome proliferator⁃activated receptor γ 2 (PPAR γ 2) gene polymorphism and the onset of sporadic Alzheimer's disease (SAD). Methods According to the method of case ⁃ control study, DNA blood specimens were randomly collected in SAD patients and controls. Polymerase chain reaction (PCR) was performed to proliferate PPARγ2 gene exon B, and sequential method was used to scan the mutational site. Polymorphism in exon B of PPAR γ 2 gene of 310 SAD patients (AD group) and 289 healthy controls (control group) in Chinese Han population were genotyped by PCR ⁃ restriction fragment length polymorphism (RFLP). All subjects were genotyped for apolipoprotein E (ApoE) by the methods previously described. Allelic and genotypic distributions in AD group and control group were compared to study the association between the polymorphism and the risk for SAD by Chi⁃square test. Results Pro12Ala (rs1801282) polymorphism in exon B of PPAR γ 2 gene was detected in the Chinese Han population by direct sequencing. The distribution of the genotype and allele frequencies of rs1801282 all coincided with Hardy ⁃ Weinberg equilibrium in SAD patients and controls. There were no significant differences of genotype or allele frequencies in SAD patients between those in controls (P = 0.647, 0.501, respectively). Among those ≥ 75 years old and ApoEε4 allelic gene carrier, the Pro/Ala gene frequency and Ala allelic gene frequnecy in AD group were all higher than those in control group, but the differences were not significant (P > 0.05, for all). There was no association between Pro12Ala polymorphism and SAD after gender, age and ApoE adjustment by Logistic regression (OR = 1.100, 95% CI: 0.580-2.090; P = 0.767). Conclusion This study does not support the association of Pro12Ala polymorphism with SAD in Chinese Han population.

DOI：10.3969/j.issn.1672-6731.2010.02.012