Efficacy of repetitive transcranial magnetic stimulation treatment with MPTP Parkinson's disease mice

Abstract

Objective To observe the efficacy of the treatment of repetitive transcranial magnetic stimulation (rTMS) on the 1 ⁃ methyl ⁃ 4 ⁃ phenyl ⁃ 1, 2, 3, 6 ⁃ tetrahydropyridine (MPTP) mouse model of Parkinson's disease (PD). Methods Fifty⁃six C57BL/6J male mice were divided into 4 groups: PD model group, sham magnetic stimulation group (s⁃rTMS group), rTMS group and control group. Mice were given intraperitoneal injection of MPTP (15 mg/kg, every 2 h, 4 times) to prepare mouse model of Parkinson's disease 24 h before accepting repetitive transcranial magnetic stimulation. The stimulation were controlled at 1.00 Hz, 1.00 T, and lasted for 25 s, being performed at fixed time for 14 d. At the 1, 3, 7 and 14 d after MPTP injection, Rotarod Test were performed to evaluate physical coordination ability and freedom of movement of mice. Immunohistochemical staining was used in the midbrain and striatum to count the number of tyrosine hydroxylase (TH) ⁃ positive cells, and high ⁃ performance liquid chromatography ⁃ electrochemical detection (HPLC ⁃ ECD) of striatal dopamine content was applyed. Results The rotary number of mice in rTMS group（85.89±3.74）were significantly higher than that in PD group (59.71 ± 8.33; P = 0.000). TH⁃positive neurons of substantia nigra in rTMS group (36.67 ± 3.82) were significantly more than PD group (31.67 ± 3.35; P = 0.000). Dopamine concentration in striatum of PD group (152.35 ± 1.64) was significantly lower than that in rTMS group (258.70 ± 1.06; P = 0.000). Conclusion The repetitive transcranial magnetic stimulation could improve the motor coordination of MPTP mice and protect injured TH ⁃ positive neurons in substantia nigra and TH ⁃ positive nerve fibers in striatum, as well as elevating dopamine level in striatum.

DOI：10.3969/j.issn.1672-6731.2011.01.014