Expressions of heme⁃oxygenase⁃1, glial fibrillary acidic protein and cyclinD1 in the perihematomal tissues in human hypertensive intracerebral hemorrhage

Abstract

Objective To investigate the relationship among the expressions of heme⁃oxygenase⁃1 (HO ⁃ 1), glial fibrillary acidic protein (GFAP) and cyclinD1 in the perihematomal tissues in human intracerebral hemorrhage (ICH) and brain renovation following ICH. Methods Paraffin⁃embedded brain tissues of 32 human fatal cases of ICH from the perihematomal tissues. The tissues from the hemorrhagic lesion side, as well as tissues from the corresponding area at the opposite side as controls, were stained with HE and immunohistochemistry staining. The expressions of HO ⁃ 1, GFAP and cyclinD1 in the perihematomal tissues were analysed with SPSS 11.5 software. Results Expression of HO ⁃ 1 could be detected in the specimens of the 2 h group [(5.30 ± 1.00)/HP]. The GFAP and cyclinD1 positive astrocytes in the perihematomal tissues were also identified at 2 h group [(22.60 ± 1.40)/HP, (11.50 ± 1.20)/HP, respectively]. All of their levels decreased gradually after the peak. The positive astrocytes of hemorrhagic lesion side at different time points were higher than contralateral expression in normal brain tissues. The difference was statistically significant. Conclusion Expressions of HO ⁃ 1, GFAP and cyclinD1 in the perihematomal tissues were "parabola". The increased expressions of HO⁃1 and cyclinD1 after ICH provide a potential protection or destruction mechanism after ICH in human.

DOI：10.3969/j.issn.1672-6731.2011.02.014