Analysis of ApoE and SLCO1B1 genetic polymorphisms of patients with Han nationality in Hebei region of China

Abstract

Objective To analyze the distribution of ApoE and SLCO1B1 genetic polymorphisms of patients with cardiovascular and cerebrovascular diseases in Han nationality of Hebei region. Methods Patients with cardiovascular and cerebrovascular diseases admitted to our hospital corresponding department from June 2019 to October 2020 were selected. The polymorphisms of ApoE and SLCO1B1 genes in 4193 patients with cardiovascular and cerebrovascular diseases were detected qualitatively by polymerase chain reaction (PCR)⁃fluorescence probe technique, to analyze the distribution characteristics of polymorphism and compare the genotype distribution difference between male and female. Results The observed mutation frequencies of ApoE and SLCO1B1 gene polymorphism of Han nationality in Hebei region were consistent with the Hardy⁃Weinberg genetic balance. Moreover, there was no significant difference in the distribution of ApoE gene polymorphism at 388T>C and 526C>T and SLCO1B1 gene polymorphism at 388A>G and 521T>C between men and women (P>0.05). Among the six genotypes of ApoE in Han nationality people, genotype ɛ3/ɛ3 accounted for 68.57% (2875/4193), followed by ɛ3/ɛ4 (15.48%, 649/4193), ɛ2/ɛ3 (12.81%, 537/4193), ɛ2/ɛ4 (1.69%, 71/4193), ɛ4/ɛ4 (0.98%, 41/4193), and ɛ2/ɛ2 (0.48%, 20/4193). There was no significant difference in the distribution of ApoE gene phenotypes between male and female (P=0.223). Among the seven genotypes of SLCO1B1 gene, *1b/*1b and *1a/*1b accounted for the largest proportion 39.57% (1659/4193) and 31.05% (1302/4193), while other genotypes *1b/*15, *1a/*1a, *1a/*15, *15/*15 and *1a/*5 accounted for 14.31% (600/4193), 7.20% (302/4193), 6.42% (269/4193), 1.43% (60/4193) and 0.02% (1/4193) respectively. There was no significant difference in the distribution of SLCO1B1 gene phenotypes between male and female (P=0.078). Conclusions The distribution of ApoE and SLCO1B1 gene polymorphisms is different in patients with cardiovascular and cerebrovascular diseases in Han nationality of Hebei region, and the distribution of ApoE and SLCO1B1 genetic polymorphisms is independent of gender.

doi：10.3969/j.issn.1672⁃6731.2021.05.012