Clinical study of intercellular adhesion molecule⁃1 expression in endothelial⁃derived exosome in children with intracranial cavernous hemangioma with epilepsy

Abstract

Objective To investigate the expression and mechanism of exosomal intercellular adhesion molecule ⁃ 1 (ICAM ⁃ 1) in patients with intracranial cavernous hemangioma (CCM) associated epilepsy. Methods Clinical data of children with epilepsy diagnosed from February 2014 to February 2019 were collected and divided into epilepsy group and CCM group according to the presence or absence of CCM. Healthy children who underwent physical examination during the same period were used as the control group. There were 20 cases in each group. The secretions in serum were sorted by magnetic beads, and ICAM⁃1 expression was detected by flow cytometry. Serum ICAM⁃1 was detected by enzyme⁃linked immunsorbent assay (ELISA). The expression of ICAM ⁃ 1 and the number of CD68⁺ macrophages were detected by immunohistochemistry. Results The results of ELISA showed that there was no significant difference in serum ICAM⁃1 concentration among the control group, epilepsy group and CCM group (P ＞ 0.05). There was no significant difference in the average fluorescence intensity of CD31⁺ exosome ICAM⁃1 between the control group and the epilepsy group. Compared with the epilepsy group, the average fluorescence intensity of CD31⁺ exosome ICAM⁃1 in the CCM group increased by 85.60% [(24.77 ± 3.90)% vs. (45.97 ± 5.06)% ; t = 3.317, P = 0.008]. The average optical density of ICAM ⁃ 1 in CCM tissue was positively correlated with the number of macrophages (r_s = 0.909, P = 0.001). The results of co ⁃ culture experiment showed that compared with the control group, the average fluorescence intensity of ICAM⁃1 on the exosome surface of co⁃culture group increased by 25.61% [(164.81 ± 7.00)% vs. (207.03 ± 9.18)%; t = 3.652, P = 0.004]; the average fluorescence intensity of ICAM ⁃ 1 on the exosome surface of LPS group further increased by 71.13% [(354.31 ± 18.22)% vs. (207.03 ± 9.18)%; t = 7.212, P = 0.000]; the blockade of IL⁃6 (t = 4.570, P = 0.001) and TNF⁃α (t = 5.105, P = 0.000) could partly reduce the expression of ICAM⁃1 on the exosome. Conclusions The expression of CD31⁺ exosome ICAM ⁃1 is significantly increased in patients with CCM associated epilepsy. There is a strong correlation between the expression of macrophages and endothelial cell⁃derived exosome ICAM⁃1.

DOI:10.3969/j.issn.1672⁃6731.2020.11.013