Possible association between polymorphisms of VEGFR2 rs2071559 and glioma risk in Chinese population

Abstract

Objective To investigate the potential association between vascular endothelial growth factor receptor 2 (VEGFR2) polymorphisms and risk of glioma in Chinese population.  Methods In this study, blood samples and clinical materials were collected from 504 patients with glioma and 527 gender- and age-matched controls, and epidemiological questionnaire surveys were conducted on them. DNA was extracted from collected blood samples and VEGFR2 rs2071559 genotyping was performed with MassARRAY Sequenom single nucleotide polymorphism (SNP) time-of-flight mass spectra chip system. HaploView 4.1 was used to test Hardy-Weinberg equilibrium (HWE) and SPSS 17.0 was used for single locus analysis.  Results The results of SNP genotyping showed that the genotyping ratio of VEGFR2 rs2071559 was 99.70%. HWE suggested that the genotype frequency of the controls was in balance (P = 0.451) and was representative of the population. The analysis of allele frequency showed that C allele of VEGFR2 rs2071559 was risk allele of glioma. It could increase the risk of glioma (OR = 1.424, 95%CI: 1.186-1.710; P = 0.000). The analysis of genotype suggested that individuals with VEGFR2 rs2071559 CT or CC genotype showed increased risk of glioma (adjusted OR = 1.407, 95% CI: 1.071-1.847, P = 0.014; adjusted OR = 1.947, 95%CI: 1.294-2.928, P = 0.001).  Conclusions This study indicated that CT and CC genotypes or C allele of VEGFR2 rs2071559 were associated with increased risk of glioma in Chinese population. The role of VEGFR2 rs2071559 polymorphism in glioma susceptibility needs further investigation.

doi: 10.3969/j.issn.1672-6731.2014.11.016